The Institute
develops research on the biology, physiology, genetics and agronomy
of olive and its cultivation and the collection
of the germplasm of Olea europaea L. to preserve the genetic
variability of the species for the purpose of clonal selection, propagation
and plant breeding planning. The collection consists of about 1000 accessions
and is one of the more extensive collections in the world
In the agronomy and plant breeding
program:
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study of varietal discrimination by analyzing polymorphism
using the RAPD technique (Random Amplified Polymorphic DNA);
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study of new agronomic techniques to increase the
quantitative and qualitative productivity of the oil as well as reducing
crop costs;
-
study of the biosynthesis of triglycerides by analyzing
the enzyme activity involved in lipid biosynthesis in the fruit and
the quality parameters of the oil.
For the environmental biology program:
For the biotechnological and "in vitro"
plant improvement programme:
-
study on "in vitro" micropropagation techniques
of olive cultivars with particular reference to the stabilization
of explants and the growth media to define an applicative technique;
-
study of the morphogenesis in somatic tissue, in particular
from petioles and leaf disks; engineered agrobacteria are used for
genetic transformation;
-
study of "in vitro" embryo culture of embryos
from olive cultivars in order to accelerate germination in both mature
and immature embryos;
-
study of cryoconservation of olive germplasm by using
cell and explant cultures;
-
construction of a genetic linkage map for the olive
species using RFLP, AFLP, RAPD and isozyme markers;
-
identification and expression study of genes related
to the oil synthesis and ripening process.
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